Oz-Enhanced Spider (Earth-1600)

Peter Parker got his powers from being bitten by a genetically-engineered spider, which was injected with the prototype Oz formula, giving him the proportionate strength and abilities of a normal spider.

Origin
Norman Osborn secretly hired the scientists of Empire State University to create a gene-enhancing formula similar to the Super-Soldier Serum. They did so and injected it into various spiders to see the results, which turned out to be a success. These "super-spiders" were displayed for a tour of students from Midtown High, who were on a field trip. One of the spiders escaped and bit one of the students, Peter Parker, which resulted  the genetic code in the spider's venom rewriting Peter's DNA, giving him spider-related powers. Norman would later rename the serum the Oz formula and injected himself with it, resulting in turning him mad as a side effect and becoming the Green Goblin.